LOCUS       sequence-77640-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mEos2-Tub1+3'UTR::URA3
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..740)
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gatctagcct ggacgggcaa atcgaacata ccattaatac taataacttc tctcatggat
       61 cctcgtctgg cattgtcagg caatccagaa tgagcaacag catgctcata cagcttaacc
      121 ttgttgtaat ctttgtcatg gcttaaaatc tcaatgcagt ggtccacaaa gtggtagcct
      181 ggtaacttga cacccttctc cttagctttg taagtagttc tgaagtcaca tcggtaatgg
      241 gcatttcctt caagcaacaa agccatatga atatcacccg tcagcactcc atcacgcaca
      301 tacattttct cagtggaggg ctcccatttc agcgtcttct tctgcataac tggaccattg
      361 gcgggaaagt tggtaccata aaatcgaact ttattataga aagtgtcccc ttccattgtt
      421 atgtcgtttc tggcaatgca aatgcccccg tcttcgaaag tcaagcttcg ttcccacgaa
      481 taccccttag gaaacgactg cttaaaatag tcttgtatgt tgtctggata tttggcgaat
      541 accctgttgc cgtaatggaa tgcagtggtc aggatatcaa aggcaaaagg cagaggtccg
      601 ccctctttga cttcaagatc catactctgt tttccctcaa aaggcttgcc tgtaccatct
      661 ccgtcgatca caaagtggtg cccgtttacg ttgccttcca tacggagttt gatcttcatg
      721 tctggcttaa tcgcactcat ctcgagcttt gccttcgttt atcttgcctg ctcatttttt
      781 agtatattct tcgaagaaat cacattactt tatataatgt ataattcatt a
//
