LOCUS       sequence-77643-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:Venus-Tub1+3'UTR::TRP1
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..777)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 agatctagcc tggacgggca aatcgaacat accattaata ctaataactt ctctcatgga
       61 tcctttgtac aattcatcca taccatgggt aataccagca gcagtaacaa attctaacaa
      121 gaccatgtgg tctctctttt cgtttggatc tttggataag gcagattgat aggataagta
      181 atggttgtct ggtaacaaga ctggaccatc accaattgga gtattttgtt gataatggtc
      241 agctaattga acaccaccat cttcaatgtt gtgtctaatt ttgaagttag ctttgatacc
      301 attcttttgt ttgtcagcag tgatgtaaac attgtgagag ttatagttgt attccaattt
      361 gtgacctaaa atgttaccat cttctttaaa atcaatacct tttaattcga ttctattaac
      421 taaggtatca ccttcaaact tgacttcagc tctggtcttg tagttaccgt catctttgaa
      481 aaaaatagtt ctttcttgaa cataaccttc tggcatggca gacttgaaaa agtcatgttg
      541 tttcatatga tctgggtatc tagcaaaaca ttgcaaacca taacctaaag tagtgactaa
      601 ggttggccat ggaactggca atttaccagt agtacaaatc aattttaagg tcaatttacc
      661 gtaagtagca tcaccttcac cttcaccgga gacagaaaat ttgtgaccat taacatcacc
      721 atctaattca accaaaattg ggacaacacc agtgaataat tcttcacctt tagacatgct
      781 ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt cttcga
//
