LOCUS       sequence-77644-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL50013
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     177..194
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(381..399)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        467..571
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
       61 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      121 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      181 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
      241 ggccgattca ttaatcactg tggtctcatt cgatggcatc tatgacagga ggtcaacaga
      301 tgggtggaat ggcttcaatg actggtggac agcaaatggg ataagctttg agaccacgtg
      361 gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
      421 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      481 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      541 aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt ccagaacctt
      601 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
      661 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
      721 cgatgtttga tgttatggag cagcaacgat gt
//
