LOCUS       sequence-77645-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pAGM1467
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     161..178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             353..370
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(433..451)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        519..623
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
       61 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      121 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      181 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc
      241 actctgtggt ctcatactgt ataagagctc atttttacaa caattaccaa caacaacaaa
      301 caacaaacaa cattacaatt acatttacaa ttatcgatac atgggcagca gccaccacca
      361 ccaccaccac agcagcggcg acgacgacga cgaaatgtga gaccacgaag tggctcttca
      421 gtggacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      481 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      541 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      601 aataatattg aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac
      661 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg
      721 ggtacagtct atgcctcggg cat
//
