LOCUS       sequence-77650-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc04_1609
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             379..390
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(787..804)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 attatctagg attgaaatat cagatttaac catagaatga ggataaatga tcgcgagtaa
       61 ataatattca caatgtacca ttttagtcat atcagataag cattgattaa tatcattatt
      121 gcttctacaa gctttaattt tattaattat tctgtatgtg tcgtcggcat ttatgttttt
      181 catattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
      241 tttgcgccat tcgatggcgc gccgcttcgt caggccacat agctttcttg ttctgatcgg
      301 aacgatcgtt ggctgacctg taggatcgta caggtttacg caagaaaatg gtttgttata
      361 gtcgaataaa tactagagaa agaggagaaa taccatatga atcgtaccac cgaacagcgt
      421 aatgcagaag ttatgaaaac cattggtctg ctggatcaga tgcctcgtgt tgaagttgat
      481 cacctgtttc gtgttcgtct gatgcagcgt attgaagcaa tggaagtgaa aaaaaccagc
      541 tggtcagcac tgcctggtgg tgcatttaat ccgcgtctgg catttatggc actgctgctg
      601 atgctgaata ttgcaagcgc actgatgctg tttatgcatg gtacaccgca ggcaaccggt
      661 agcagcggtg caattgcaga aagcctgacc gaagattatg gtggtccggc actgagctat
      721 tatgatgatc agaccaccat tgatcgctaa ggatccctgt tttggcggat gagagaagat
      781 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
      841 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
      901 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cct
//
