LOCUS       sequence-77651-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes yeast-optimized dCas9_VP64 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pTPGI_dCas9_VP64
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(147..167)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(207..648)
                     /label=TPGI promoter
                     /note="S. cerevisiae GAL1 promoter modified to contain two 
                     copies of the tet operator (Ellis et al., 2009)"
     primer_bind     complement(213..236)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    284..302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    305..323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..648
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ttctttttna tggagtgtcc tgatccagtg ttacccaaaa ccttgaactt cttggatgga
       61 accttgtatt catcagtaat aacagcccaa ccaacagagt ttgtaccgat agccaaacca
      121 atagagtatt tcttatcccc gggtctaact tttctcttct tctttggggc tctagacatt
      181 cttttaatcg tggatccggg gtttttctcc ttgacgttaa agtatagagg tatattaaca
      241 attttttgtt gatactttta ttacatttga ataagaagta atatctctat cactgatagg
      301 gagatctcta tcactgatag ggacagtttt tgcatttata tatctgttaa tagatcaaaa
      361 atcatcgctt cgctgctgca gtaccccaga aataaggcta aaaaagtaat cgcattatca
      421 tcctatggtt gttaatttga ttcgttcatt tgaaggtttg tggggccagg ttactgccaa
      481 tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg
      541 cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga cgcacggagg
      601 agagtcttcc ttcggagggc tgtcacccgc tcggcggctt ctaatccgta cttcgaattc
      661 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
      721 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
      781 ctctttgagc aataaagctg ataacaaaat ctttgtcgct cttcgcaacg tcaacagtac
      841 ccttagtata ttctccagta
//
