LOCUS       sequence-77653-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, 
            Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
  TITLE     Design of orthogonal genetic switches based on a crosstalk map of 
            sigmas, anti-sigmas, and promoters.
  JOURNAL   Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
  PUBMED    24169405
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-10-29"; volume: "9:702. doi"; pages: " 
            10.1038/msb.2013.58"
COMMENT     Sequence Label: pVRc04_1609
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..25
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     RBS             422..433
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa aacagggatc
       61 cttagcgatc aatggtggtc tgatcatcat aatagctcag tgccggacca ccataatctt
      121 cggtcaggct ttctgcaatt gcaccgctgc taccggttgc ctgcggtgta ccatgcataa
      181 acagcatcag tgcgcttgca atattcagca tcagcagcag tgccataaat gccagacgcg
      241 gattaaatgc accaccaggc agtgctgacc agctggtttt tttcacttcc attgcttcaa
      301 tacgctgcat cagacgaaca cgaaacaggt gatcaacttc aacacgaggc atctgatcca
      361 gcagaccaat ggttttcata acttctgcat tacgctgttc ggtggtacga ttcatatggt
      421 atttctcctc tttctctagt atttattcga ctataacaaa ccattttctt gcgtaaacct
      481 gtacgatcct acaggtcagc caacgatcgt tccgatcaga acaagaaagc tatgtggcct
      541 gacgaagcgg cgcgccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc
      601 ggaagagagt caattcaggg tggtgaatat gaaaaacata aatgccgacg acacatacag
      661 aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga
      721 tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat
      781 ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga
      841 tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc
      901 aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa
      961 agaagcgaaa acatcaggtc ttatcactgg gtttagtttc
//
