LOCUS       sequence-77655-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for DNA cleavage/repair in mammalian cells 
            expressing iRFP with selectable puromycin marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  TITLE     TLR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..212)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             complement(264..971)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(542..561)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     811..829
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat
       61 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca
      121 gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt
      181 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctctgcagct
      241 agaatcagtc cggactaatg cattcacttg tacagctcgt ccatgccgcc ggtggagtgg
      301 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
      361 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag
      421 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc
      481 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg
      541 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg
      601 tagatgaact cgccgtcctg cagagaggag tcctgggtca cggtcaccac gccgccgtcc
      661 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg
      721 gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg
      781 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
      841 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
      901 ccctccatgt gcaccttgaa gcgcaggaac tccttgatga tggcggagtt atcctcctcg
      961 cccttgctca cagggcccgg attctcctcc acgtcac
//
