LOCUS       sequence-77656-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for DNA cleavage/repair in mammalian cells 
            expressing iRFP with selectable puromycin marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  TITLE     TLR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(126..179)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(356..377)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     705..724
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     970..991
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc
       61 atgtgcacct tgaagcgcag gaactccttg atgatggcgg agttatcctc ctcgcccttg
      121 ctcacagggc ccggattctc ctccacgtca ccgcatgtta gaagacttcc tctgccctca
      181 cccacgttga tcctagcgct ggcgcaagcg gcggggtgcc tgtccatgcc gctctcctgg
      241 gcgcagctca tgggcagggt gccatcgtcc tgagcggcca cctcaggggg gaagccgtgg
      301 ctggccggcc ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      361 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      421 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      481 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
      541 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      601 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      661 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      721 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      781 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
      841 ttcacactag ttagggataa cagggtaata cctgcaggtt gccggtggtg cagatgaact
      901 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
      961 ggccgtttac gtcgccgtcc agctcgacca ggatggg
//
