LOCUS       sequence-77659-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes yeast-optimized dCas9_VP64 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pTPGI_dCas9_VP64
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        125..272
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 ctatatatat aatataggaa gcatttaata gaacagcatc gtaatatatg tgtactttgc
       61 agttatgacg ccagatggca gtagtggaag atattcttta ttgaaaaata gcttgtcacc
      121 ttacgtacaa tcttgatccg gagcttttct ttttttgccg attaagaatt aattcggtcg
      181 aaaaaagaaa aggagagggc caagagggag ggcattggtg actattgagc acgtgagtat
      241 acgtgattaa gcacacaaag gcagcttgga gtatgtctgt tattaatttc acaggtagtt
      301 ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag
      361 attccgatgc tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc
      421 cggttattgc aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc
      481 cgaaatactt ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca
      541 atgattacgg cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag
      601 agttcctcgg tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac
      661 tcagtgcagc ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg
      721 ggacaggtga acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc
      781 ccgaaagctt
//
