LOCUS       sequence-77660-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for DNA cleavage/repair in mammalian cells 
            expressing iRFP with selectable puromycin marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     TLR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     130..149
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     395..416
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(459..512)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
       61 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      121 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      181 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
      241 aagcactgca cgccgtaggt cagggttcac actagttagg gataacaggg taatacctgc
      301 aggttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
      361 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      421 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacgg ggcccggatt ctcctccacg
      481 tcaccgcatg ttagaagact tcctctgccc tctctagact cttccatcac gccgatctgc
      541 caggccagaa cctgggcgac tagctcgcag gcttggcggc catcgagatc gacgtagtac
      601 ggcgttcggt gatggcaaac gatcaatccc cacagtcgct cgccgcgcaa aatcgagatc
      661 gacatcgtgc cgtgcatgcc tatgttgcgc atgaattcca gatggacggg cgagacgctg
      721 cgcaggatgg cgaagctaag atcaatcggc cgcccggtga ccggattgag gtctggggtg
      781 accggcaccg gccgataatt gatatcggga atgatccgta ccgggttgat ggtatagagc
      841 cgacgggcct gcgccggcac ggttgaggca ggatagtgca ggcctagttt tgactcgacc
      901 tcggcgcacc gatcctctgc gatcacttcg ccgctgaagt cggaggcgaa gcgatagatc
      961 atcacccgat cgaagccggt aatcttccgc acctc
//
