LOCUS       sequence-77661-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes yeast-optimized dCas9_VP64 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pTPGI_dCas9_VP64
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..124
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(156..305)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
ORIGIN
        1 aaataacgtt cttaatacta acataactat aaaaaaataa atagggacct agacttcagg
       61 ttgtctaact ccttcctttt cggttagagc ggatgtgggg gaagggcgtg aatgtaagcg
      121 tgacataact aattacatga ctcgagttcc taggaaagca tgtctaagtc gaaatcgtcc
      181 agagcgtctg atccaagcat atccaggtca aaatcatcta aggcgtcact tccaagcatg
      241 tcgaggtcaa agtcatctag cgcatcagaa cccagcatat ccaagtcgaa gtcgtccaaa
      301 gcgtcagaac ctccaccgtc gactccagat ctaccagaac catcaccacc taattgagac
      361 aagtcgattc tagtttcgta caaaccagta atagattggt ggatcaaagt agcgtccaaa
      421 acttctttgg tagaggtgta tctctttctg tcgatagtgg tatcgaaata cttaaaagca
      481 gctggagcac ccaagttagt caaagtgaac aagtgaatga tgttttcagc ttgttctcta
      541 attggcttat ctctgtgctt gttgtaagct gacaaaactt tgtccaaatt ggcgtcagcc
      601 aaaatgactc ttttggaaaa ttcggaaatt tgttcaatga tttcgtccaa gta
//
