LOCUS       sequence-77662-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for DNA cleavage/repair in mammalian cells 
            expressing iRFP with selectable puromycin marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  TITLE     TLR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccctcactcg gcgcgccagt cctccgacag actgagtcga tcgcttcagt caagatttaa
       61 ttaacgccac catggctgaa ggatccgtcg ccaggcagcc tgacctcttg acctgcgacg
      121 atgagccgat ccatatcccc ggtgccatcc aaccgcatgg actgctgctc gccctcgccg
      181 ccgacatgac gatcgttgcc ggcagcgaca accttcccga actcaccgga ctggcgatcg
      241 gcgccctgat cggccgctct gcggccgatg tcttcgactc ggagacgcac aaccgtctga
      301 cgatcgcctt ggccgagccc ggggcggccg tcggagcacc gatcactgtc ggcttcacga
      361 tgcgaaagga cgcaggcttc atcggctcct ggcatcgcca tgatcagctc atcttcctcg
      421 agctcgagcc tccccagcgg gacgtcgccg agccgcaggc gttcttccgc cgcaccaaca
      481 gcgccatccg ccgcctccag gccgccgaaa ccttggaaag cgcctgcgcc gccgcggcgc
      541 aagaggtgcg gaagattacc ggcttcgatc gggtgatgat ctatcgcttc gcctccgact
      601 tcagcggcga agtgatcgca gaggatcggt gcgccgaggt cgagtcaaaa ctaggcctgc
      661 actatcctgc ctcaaccgtg ccggcgcagg cccgtcggct ctataccatc aacccggtac
      721 ggatcattcc cgatatcaat tatcggccgg tgccggtcac cccagacctc aatccggtca
      781 ccgggcggcc gattgatctt agcttcgcca tcctgcgcag cgtctcgccc gtccatctgg
      841 aattcatgcg caacataggc atgcacggca cgatgtcgat ctcgattttg cgcggcgagc
      901 gactgtgggg attgatcgtt tgccatcacc gaacgccgta ctacgtcgat ctcgatggcc
      961 gccaagcctg cgagctagtc gcccaggttc tggcctg
//
