LOCUS       sequence-77663-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the pentahistidine-tagged catalytic domain of bovine 
            enteropeptidase in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Skala W, Goettig P, Brandstetter H
  TITLE     Do-it-yourself histidine-tagged bovine enterokinase: A handy member 
            of the protein engineer's toolbox.
  JOURNAL   J Biotechnol. 2013 Dec;168(4):421-5. doi: 
            10.1016/j.jbiotec.2013.10.022. Epub 2013 Oct 30.
  PUBMED    24184090
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiotec.2013.10.022"; journalName: "J Biotechnol"; date: 
            "2013-12"; volume: "168"; issue: "4"; pages: "421-5"
COMMENT     Sequence Label: pET-15b-EK_C122S_His5
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(750..764)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     RBS             783..805
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    820..844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(844..863)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(845..863)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgggctttgt tagcagccgg atcctcgagc taatgatgat gatgatgtag aaaactttgt
       61 atccactctg tgaaccttgg gacccgggca tacacccctg ggcgattagg cagtgcacat
      121 tgatatccaa atgacgtcac gccagccagg agccatctgt tgttttcttg gcacatgagt
      181 ggtccgcctg aatccccctg acaagaatct acccctcctg catcatagcc tgcacacacc
      241 atattttccg taatgttata ttctggcatc tgttgttgac atttctcatt tgatagaagg
      301 ggaacgtcag cttcttgcag tacgtctgca gtagaacctt gatatataag tgccccccag
      361 ccagcaatag aacaaattct tcctggggga aaaacttgat tttcttctgg taagctaata
      421 ggctgtatat aatctgtgta gttcactttc atttcaagat gcatcatggc aatgtcattg
      481 ttctttctcc gtttattgta gtgtcggttt atgacaattt ggtcaatcaa cctagtttct
      541 atctgaggag aagtcagatt tgatgccata tgcaggccta gcactgcttt ccacttagac
      601 ggctccatat ttctcccgta cacgcagtgg gcggccgaca ccagccaatc cctgctcacc
      661 agagaagctc cgcagacctg ttgatcgtcg aaatacagag caacgaccca aggccaggct
      721 ccttctctgg agtcacttcc tccgacaatc ttgtcgtcgt catctatagg gcccatggta
      781 tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa
      841 ttcccctata gtgagtcgta ttaat
//
