LOCUS       sequence-77664-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for DNA cleavage/repair in mammalian cells 
            expressing iRFP with selectable puromycin marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  TITLE     TLR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..653)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(137..157)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     634..653
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(669..880)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgacacta gattcaggca
       61 ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg
      121 acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag
      181 gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga
      241 cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg
      301 ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc
      361 aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc
      421 gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc
      481 ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccggg
      541 tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc
      601 cgggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtggct
      661 cgaggatcct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact
      721 gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca
      781 ctttcccagt ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc gacccctccc
      841 cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcct
      901 ctgcagctag aatcagtccg gactaatgca ttcacttgta cagctcgtcc atgccgccgg
      961 tggagtggcg gccctcggcg cgttcgtact g
//
