LOCUS       sequence-77665-e        6649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bi-directional expression vector for yeast, galactose inducible, 
            LEU2 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Miller CA 3rd, Martinat MA, Hyman LE
  TITLE     Assessment of aryl hydrocarbon receptor complex interactions using 
            pBEVY plasmids: expressionvectors with bi-directional promoters for 
            use in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.
  PUBMED    9671822
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998-08-1"; volume: "26(15)"; pages: "3577-83"
COMMENT     Sequence Label: pBEVY-GL
FEATURES             Location/Qualifiers
     source          1..6649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(267..454)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     375..399
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        624..1288
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    840..957
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1252..1275
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      1325..1624
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      1649..2995
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3133..4227)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4239..4637)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(4657..4675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4743..4847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4848..4916,4917..5708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5066..5085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5879..6467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6368..6387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6621..6638
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttt
      241 catagggtag gggaatttcg ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat
      301 gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaac aagaattttc
      361 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt
      421 taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc
      481 taccaacgat ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga
      541 caaccttgat tggagacttg accaaacctc tggcgaagaa gtccaaagct tgcatgcctg
      601 caggtcgact ctagaggatc ccctttcaaa aattcttact ttttttttgg atggacgcaa
      661 agaagtttaa taatcatatt acatggcatt accaccatat acatatccat atacatatcc
      721 atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct tagcctaaaa
      781 aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat attgaagtac
      841 ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc
      901 tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac
      961 aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg gcagtaacct
     1021 ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt
     1081 agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta
     1141 acagatatat aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg
     1201 tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct
     1261 ctatacttta acgtcaagga gaaaaaaccc gggtaccgag ctcgaattcg acacttctaa
     1321 ataagcggat ctcctatgcc ttcacgattt atagtttcca ttatcaagta tgcctatatt
     1381 agtatatagc atctttagat gacagtgttc gaagtttcac gaataaaaga taatattcta
     1441 ctttttgctc ccaccgcgtt tgctagcacg agtgaacacc atccctcgcc tgtgagttgt
     1501 acccattcct ctaaactgta gacatggtag cttcagcagt gttcgttatg tacggcatcc
     1561 tccaacaaac agtcggttat agtttgtcct gctcctctga atcgtctccc tcgatatttc
     1621 tcattttcct tcgcatgccc tcgagcggaa cgaagcatct gtgcttcatt ttgtagaaca
     1681 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     1741 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     1801 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     1861 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     1921 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     1981 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     2041 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     2101 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     2161 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     2221 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     2281 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     2341 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     2401 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     2461 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     2521 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
     2581 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gctagagaat
     2641 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
     2701 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
     2761 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
     2821 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
     2881 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
     2941 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcgatcc
     3001 gatgataagc tgtcaaacat gagaattaat tctaccctat gaacatattc cattttgtaa
     3061 tttcgtgtcg tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa
     3121 aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc
     3181 ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt
     3241 aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat
     3301 tgcagcagac aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga
     3361 accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga
     3421 cgcagatggc aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc
     3481 accaaacatg ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat
     3541 catggcggca gaatcaatca attgatgttg aaccttcaat gtaggaaatt cgttcttgat
     3601 ggtttcctcc acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa
     3661 ggaccaaata ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat
     3721 tctttgcact tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc
     3781 ttcctttctc ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt
     3841 acctttagca aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt
     3901 acatggtctt aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc
     3961 aggtctaaca ctacctgtac cccatttagg accacccaca gcacctaaca aaacggcatc
     4021 aaccttcttg gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc
     4081 agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat
     4141 agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa
     4201 aacgacgatc ttcttagggg cagacatagg ggcagacatt agaatgtata tccttgaaat
     4261 atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaataagac gattgctaac
     4321 cacctattgg aaaaaacaat aggtccttaa ataatattgt caacttcaag tattgtgatg
     4381 caagcattta gtcatgaacg cttctctatt ctatatgaaa agccggttcc ggcctctcac
     4441 ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgcggcga cctctgaaat
     4501 taacaaaaaa tttccagtca tcgaatttgt ctgtgcgata gcgcccctgt gtgttctcgt
     4561 tatgttgagg aaaaaaataa tggttgctaa gagattcgaa ctcttgcatc ttacgatacc
     4621 tgagtattcc cacagttaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     4681 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     4741 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4801 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4861 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4921 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4981 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5041 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5101 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5161 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5221 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5281 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5341 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5401 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5461 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5521 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5581 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5641 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5701 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5761 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5821 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5881 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5941 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6001 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6061 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6121 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6181 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6241 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6301 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6361 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6421 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6481 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6541 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6601 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaga
//
