LOCUS       sequence-77672-e        6534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bi-directional expression vector for yeast, constitutively active, 
            URA3 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Miller CA 3rd, Martinat MA, Hyman LE
  TITLE     Assessment of aryl hydrocarbon receptor complex interactions using 
            pBEVY plasmids: expressionvectors with bi-directional promoters for 
            use in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.
  PUBMED    9671822
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998-08-1"; volume: "26(15)"; pages: "3577-83"
COMMENT     Sequence Label: pBEVY-U
FEATURES             Location/Qualifiers
     source          1..6534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(267..454)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     375..399
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(623..1268)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(708..731)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     promoter        1277..1673
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      1712..2011
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      2036..3382
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3493..4296)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4297..4517)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(4542..4560)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4628..4732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4733..4801,4802..5593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4951..4970)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5764..6352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6253..6272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6506..6523
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttt
      241 catagggtag gggaatttcg ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat
      301 gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaac aagaattttc
      361 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt
      421 taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc
      481 taccaacgat ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga
      541 caaccttgat tggagacttg accaaacctc tggcgaagaa gtccaaagct tgcatgcctg
      601 caggtcgact ctagaggatc cgtcgaaact aagttcttgg tgttttaaaa ctaaaaaaaa
      661 agactaacta taaaagtaga atttaagaag tttaagaaat agatttacag aattacaatc
      721 aatacctacc gttctttata tacttattag tcaagtaggg gaataatttc agggaactgg
      781 tttcaacctt ttttttcagc tttttccaaa tcagagagag cagaaggtaa tagaaggtgt
      841 aagaaaatga gatagataca tgcgtgggtc aattgccttg tgtcatcatt tactccaggc
      901 aggttgcatc actccattga ggttgtgccc gttttttgcc tgtttgtgcc cctgttctct
      961 gtagttgcgc taagagaatg gacctatgaa ctgatggttg gtgaagaaaa caatattttg
     1021 gtgctgggat tctttttttt tctggatgcc agcttaaaaa gcgggctcca ttatatttag
     1081 tggatgccag gaataaactg ttcacccaga cacctacgat gttatatatt ctgtgtaacc
     1141 cgccccctat tttgggcatg tacgggttac agcagaatta aaaggctaat tttttgacta
     1201 aataaagtta ggaaaatcac tactattaat tatttacgta ttctttgaaa tggcagtatt
     1261 gataatgata aactcccgga ttcttttctt tttttttctt ttctctctcc cccgttgttg
     1321 tctcaccata tccgcaatga caaaaaaaat gatggaagac actaaaggaa aaaattaacg
     1381 acaaagacag caccaacaga tgtcgttgtt ccagagctga tgaggggtat cttcgaacac
     1441 acgaaacttt ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg
     1501 ccttccttcc agttacttga atttgaaata aaaaaagttt gccgctttgc tatcaagtat
     1561 aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc
     1621 cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca actccccggg
     1681 taccgagctc gaattcgaca cttctaaata agcggatctc ctatgccttc acgatttata
     1741 gtttccatta tcaagtatgc ctatattagt atatagcatc tttagatgac agtgttcgaa
     1801 gtttcacgaa taaaagataa tattctactt tttgctccca ccgcgtttgc tagcacgagt
     1861 gaacaccatc cctcgcctgt gagttgtacc cattcctcta aactgtagac atggtagctt
     1921 cagcagtgtt cgttatgtac ggcatcctcc aacaaacagt cggttatagt ttgtcctgct
     1981 cctctgaatc gtctccctcg atatttctca ttttccttcg catgccctcg agcggaacga
     2041 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     2101 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     2161 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     2221 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     2281 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     2341 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     2401 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     2461 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     2521 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     2581 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     2641 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     2701 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     2761 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     2821 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     2881 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     2941 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     3001 agttcctata ctttctagct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     3061 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     3121 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     3181 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     3241 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     3301 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
     3361 tcatttcctt tgatattgga tcgatccgat gataagctgt caaacatgag aattgggtaa
     3421 taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta
     3481 taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt
     3541 tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac
     3601 aataataatg tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa
     3661 tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc
     3721 atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt
     3781 cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa
     3841 ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa
     3901 accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc
     3961 tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt
     4021 tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt
     4081 gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg
     4141 acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca
     4201 caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg
     4261 agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcatgt
     4321 ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca
     4381 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
     4441 gattaccgaa tcaaaaaaat ttcaaagaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
     4501 tgatgaattg aattgaaaag ctaattcttg aagacgaaag ggcctcgtga tacgcctatt
     4561 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4621 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4681 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4741 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4801 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4861 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4921 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4981 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5041 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5101 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5161 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5221 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5281 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5341 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5401 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5461 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5521 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5581 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5641 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5701 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5761 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5821 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5881 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5941 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6001 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6061 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6121 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6181 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6241 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6301 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6361 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6421 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6481 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga
//
