LOCUS       sequence-77674-e        6282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bi-directional expression vector for yeast, constitutively active, 
            TRP1 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Miller CA 3rd, Martinat MA, Hyman LE
  TITLE     Assessment of aryl hydrocarbon receptor complex interactions using 
            pBEVY plasmids: expressionvectors with bi-directional promoters for 
            use in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 1998 Aug 1;26(15):3577-83.
  PUBMED    9671822
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998-08-1"; volume: "26(15)"; pages: "3577-83"
COMMENT     Sequence Label: pBEVY-T
FEATURES             Location/Qualifiers
     source          1..6282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(267..454)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     375..399
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        complement(623..1268)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(708..731)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     promoter        1277..1673
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     terminator      1712..2011
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     rep_origin      2036..3382
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3494..4168)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(4169..4270)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(4290..4308)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4376..4480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4481..4549,4550..5341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4699..4718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5512..6100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6001..6020
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6254..6271
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctttt
      241 catagggtag gggaatttcg ggcatgccgg tagaggtgtg gtcaataaga gcgacctcat
      301 gctatacctg agaaagcaac ctgacctaca ggaaagagtt actcaagaac aagaattttc
      361 gttttaaaac ctaagagtca ctttaaaatt tgtatacact tatttttttt ataacttatt
      421 taataataaa aatcataaat cataagaaat tcgcttattt agaagtgtca acaacgtatc
      481 taccaacgat ttgacccttt tccatctttt cgtaaatttc tggcaaggta gacaagccga
      541 caaccttgat tggagacttg accaaacctc tggcgaagaa gtccaaagct tgcatgcctg
      601 caggtcgact ctagaggatc cgtcgaaact aagttcttgg tgttttaaaa ctaaaaaaaa
      661 agactaacta taaaagtaga atttaagaag tttaagaaat agatttacag aattacaatc
      721 aatacctacc gttctttata tacttattag tcaagtaggg gaataatttc agggaactgg
      781 tttcaacctt ttttttcagc tttttccaaa tcagagagag cagaaggtaa tagaaggtgt
      841 aagaaaatga gatagataca tgcgtgggtc aattgccttg tgtcatcatt tactccaggc
      901 aggttgcatc actccattga ggttgtgccc gttttttgcc tgtttgtgcc cctgttctct
      961 gtagttgcgc taagagaatg gacctatgaa ctgatggttg gtgaagaaaa caatattttg
     1021 gtgctgggat tctttttttt tctggatgcc agcttaaaaa gcgggctcca ttatatttag
     1081 tggatgccag gaataaactg ttcacccaga cacctacgat gttatatatt ctgtgtaacc
     1141 cgccccctat tttgggcatg tacgggttac agcagaatta aaaggctaat tttttgacta
     1201 aataaagtta ggaaaatcac tactattaat tatttacgta ttctttgaaa tggcagtatt
     1261 gataatgata aactcccgga ttcttttctt tttttttctt ttctctctcc cccgttgttg
     1321 tctcaccata tccgcaatga caaaaaaaat gatggaagac actaaaggaa aaaattaacg
     1381 acaaagacag caccaacaga tgtcgttgtt ccagagctga tgaggggtat cttcgaacac
     1441 acgaaacttt ttccttcctt cattcacgca cactactctc taatgagcaa cggtatacgg
     1501 ccttccttcc agttacttga atttgaaata aaaaaagttt gccgctttgc tatcaagtat
     1561 aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc
     1621 cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca actccccggg
     1681 taccgagctc gaattcgaca cttctaaata agcggatctc ctatgccttc acgatttata
     1741 gtttccatta tcaagtatgc ctatattagt atatagcatc tttagatgac agtgttcgaa
     1801 gtttcacgaa taaaagataa tattctactt tttgctccca ccgcgtttgc tagcacgagt
     1861 gaacaccatc cctcgcctgt gagttgtacc cattcctcta aactgtagac atggtagctt
     1921 cagcagtgtt cgttatgtac ggcatcctcc aacaaacagt cggttatagt ttgtcctgct
     1981 cctctgaatc gtctccctcg atatttctca ttttccttcg catgccctcg agcggaacga
     2041 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     2101 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     2161 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     2221 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     2281 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     2341 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     2401 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     2461 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     2521 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     2581 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     2641 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     2701 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     2761 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     2821 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     2881 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     2941 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     3001 agttcctata ctttctagct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     3061 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     3121 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     3181 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     3241 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     3301 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
     3361 tcatttcctt tgatattgga tcgatccgat gataagctgt caaacatgag aattgatctt
     3421 ttatgcttgc ttttcaaaag gcttgcaggc aagtgcacaa acaatactta aataaatact
     3481 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt
     3541 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa
     3601 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctctcgg
     3661 ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc
     3721 cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga
     3781 gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact
     3841 cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat
     3901 tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg
     3961 gagtgcctga actattttta tatgctttta caagacttga aattttcctt gcaataaccg
     4021 ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat
     4081 ctagtgcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag
     4141 aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt
     4201 atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt
     4261 cgaccgaatt aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     4321 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     4381 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4441 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4501 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4561 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4621 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4681 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4741 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4801 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4861 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4921 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4981 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5041 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5101 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5161 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5221 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5281 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5341 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     5401 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5461 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5521 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5581 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5641 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5701 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5761 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5821 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5881 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5941 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6001 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6061 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6121 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6181 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6241 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
