LOCUS       sequence-77678-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:Venus-Tub1+3'UTR::HIS3
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..776)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gatctagcct ggacgggcaa atcgaacata ccattaatac taataacttc tctcatggat
       61 cctttgtaca attcatccat accatgggta ataccagcag cagtaacaaa ttctaacaag
      121 accatgtggt ctctcttttc gtttggatct ttggataagg cagattgata ggataagtaa
      181 tggttgtctg gtaacaagac tggaccatca ccaattggag tattttgttg ataatggtca
      241 gctaattgaa caccaccatc ttcaatgttg tgtctaattt tgaagttagc tttgatacca
      301 ttcttttgtt tgtcagcagt gatgtaaaca ttgtgagagt tatagttgta ttccaatttg
      361 tgacctaaaa tgttaccatc ttctttaaaa tcaatacctt ttaattcgat tctattaact
      421 aaggtatcac cttcaaactt gacttcagct ctggtcttgt agttaccgtc atctttgaaa
      481 aaaatagttc tttcttgaac ataaccttct ggcatggcag acttgaaaaa gtcatgttgt
      541 ttcatatgat ctgggtatct agcaaaacat tgcaaaccat aacctaaagt agtgactaag
      601 gttggccatg gaactggcaa tttaccagta gtacaaatca attttaaggt caatttaccg
      661 taagtagcat caccttcacc ttcaccggag acagaaaatt tgtgaccatt aacatcacca
      721 tctaattcaa ccaaaattgg gacaacacca gtgaataatt cttcaccttt agacatgctt
      781 tgccttcgtt tatcttgcct gctcattttt tagtatattc ttcgaaga
//
