LOCUS       sequence-77679-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mRuby2-Tub1+3'UTR::HIS3
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..773)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gatctagcct ggacgggcaa atcgaacata ccattaatac taataacttc tctcatggat
       61 cccttgtaca gctcgtccat cccaccacca agcccggcga acttggcaac tgcgtgttcg
      121 cgttgtacta cgaacatttc attgtcactt tcctctaacc tttccaggcg gtgatcaacg
      181 gcatggatac cgggcatctt gatgttcccg acggtctttt ttgacctgta agttgttacg
      241 aaagagcaag acagatggcc accaccatca actttcagtg ccatatgagt gtatcccctc
      301 agaccaccat ctgctggata catcatctct gtattaggct cccaaccctt ggtcttcttc
      361 tgcatcacgg gaccattgga gggaaagttt acccctctga cttggacgtg gtaaacgaga
      421 cagccatcct caaggctggt gtcctgcatg acggtgacga ctccaccatc ttcgtatctc
      481 gtaactcttt cccaagtaaa accctcagga aaggactgtt taaagaaatc aggaatgcct
      541 ttcgggtact tgataaaagt acggctgcca tacatgaacg acgtggcaag aatgtcaaag
      601 gcaaatggca ggggtcctcc ctcgatgact ttgatcctca tggtttgagt tcccatgtac
      661 ggattgcctt ctccttcacc tgtgcatttg aattggtggc cgttgaccga accttccatg
      721 accaccttca tacgcatatt ttccttgatc agctcttcgc ccttagacac catgctttgc
      781 cttcgtttat cttgcctgct cattttttag tatattcttc gaagaaatca cattacttta
      841 tataatgtat aattcattat gtgataatg
//
