LOCUS       sequence-77681-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of 
            human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..82)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(133..166)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             180..206
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat
       61 agcatacatt atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct
      121 gggttaaggc aaataacttc gtataggata ctttatacga agttatgcta gccaccatgt
      181 acccatacga tgttccagat tacgctacct tgcacaataa cagtacaacc tcgcctttgt
      241 ttccaaacat cagctcctcc tggatacaca gcccctccga tgcagggctg cccccgggaa
      301 ccgtcactca tttcggcagc tacaatgttt ctcgagcagc tggcaatttc tcctctccag
      361 acggtaccac cgatgaccct ctgggaggtc ataccgtctg gcaagtggtc ttcatcgctt
      421 tcttaacggg catcctggcc ttggtgacca tcatcggcaa catcctggta attgtgtcat
      481 ttaaggtcaa caagcagctg aagacggtca acaactactt cctcttaagc ctggcctgtg
      541 ccgatctgat tatcggggtc atttcaatga atctgtttac gacctacatc atcatgaatc
      601 gatgggcctt agggaacttg gcctgtgacc tctggcttgc cattgactgc gtagccagca
      661 atgcctctgt tatgaatctt ctggtcatca gctttgacag atacttttcc atcacgaggc
      721 cgctcacgta ccgagccaaa cgaacaacaa agagagccgg tgtgatgatc ggtctggctt
      781 gggtcatctc ctttgtcctt tgggctcctg ccatcttgtt ctggcaatac tttgttggaa
      841 agagaactgt gcctccggga gagtgcttca ttcagttcct cagtgagccc accattactt
      901 ttggcacagc catc
//
