LOCUS       sequence-77686-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of 
            human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..82)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(133..166)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             180..206
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat
       61 agcatacatt atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct
      121 gggttaaggc aaataacttc gtataggata ctttatacga agttatgcta gccaccatgt
      181 acccatacga tgttccagat tacgctatgg ccaacttcac acctgtcaat ggcagctcgg
      241 gcaatcagtc cgtgcgcctg gtcacgtcat catcccacaa tcgctatgag acggtggaaa
      301 tggtcttcat tgccacagtg acaggctccc tgagcctggt gactgtcgtg ggcaacatcc
      361 tggtgatgct gtccatcaag gtcaacaggc agctgcagac agtcaacaac tacttcctct
      421 tcagcctggc gtgtgctgat ctcatcatag gcgccttctc catgaacctc tacaccgtgt
      481 acatcatcaa gggctactgg cccctgggcg ccgtggtctg cgacctgtgg ctggccctgg
      541 actgcgtggt gagcaacgcc tccgtcatga accttctcat catcagcttt gaccgctact
      601 tctgcgtcac caagcctctc acctaccctg cccggcgcac caccaagatg gcaggcctca
      661 tgattgctgc tgcctgggta ctgtccttcg tgctctgggc gcctgccatc ttgttctggc
      721 agtttgtggt gggtaagcgg acggtgcccg acaaccagtg cttcatccag ttcctgtcca
      781 acccagcagt gacctttggc acagccattg ctggcttcta cctgcctgtg gtcatcatga
      841 cggtgctgta catccacatc tccctggcca gtcgcagccg agtccacaag caccggcccg
      901 agggcccgaa
//
