LOCUS       sequence-77688-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of 
            human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..82)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(133..166)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             180..206
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat
       61 agcatacatt atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct
      121 gggttaaggc aaataacttc gtataggata ctttatacga agttatgcta gccaccatgt
      181 acccctacga cgtccccgac tacgccacct tgcacagtaa cagtacaacc tcgcctttgt
      241 ttcccaacat cagctcttcc tgggtgcaca gtccctcgga ggcagggctg cccttgggga
      301 cagtcactca gttgggcagc tacaacattt cacaagaaac tgggaatttc tcctcaaacg
      361 acacctccag cgaccctctc gggggtcaca ccatctggca agtggtcttc attgccttct
      421 taactggctt cctggcattg gtgaccatca ttggcaacat ccttgtcatt gtggccttca
      481 aggtcaacaa acagctgaag acagtcaaca actacttcct cttaagcctg gcctgtgcag
      541 acctgatcat cggggtcatt tccatgaacc tgttcactac ctacatcatt atgaaccgtt
      601 gggcactggg gaacttagcc tgcgacctct ggctctccat tgactgtgtg gccagcaatg
      661 cctctgtcat gaatctgctg gtcatcagct ttgacaggta cttttccatc acttctccat
      721 tccgctacca gagcctgatg accagggctc gagctggtgt gatgattggt ctggcttggg
      781 tcatctcctt tgtcctatgg gctcctgcca tcttgttctg gcaatacttt gtagggaaga
      841 gaactgtgcc ccc
//
