LOCUS       sequence-77692-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gs-coupled rM3D DREADD fused with mCherry under the 
            control of human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-DIO-rM3D(Gs)-mCherry
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(43..76)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(127..160)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gtaatccaga ggttgattat cgataagctt gatatcgaat tcataacttc gtatagcata
       61 cattatacga agttattctt tgcaccattc taaagaataa cagtgataat ttctgggtta
      121 aggcaaataa cttcgtatag gatactttat acgaagttat gctagcgcca ccatgacctt
      181 gcacagtaac agtacaacct cgcctttgtt tcccaacatc agctcttcct gggtgcacag
      241 tccctcggag gcagggctgc ccttggggac agtcactcag ttgggcagct acaacatttc
      301 acaagaaact gggaatttct cctcaaacga cacctccagc gaccctctcg ggggtcacac
      361 catctggcaa gtggtcttca ttgccttctt aactggcttc ctggcattgg tgaccatcat
      421 tggcaacatc cttgtcattg tggccttcaa ggtcaacaaa cagctgaaga cagtcaacaa
      481 ctacttcctc ttaagcctgg cctgtgcaga cctgatcatc ggggtcattt ccatgaacct
      541 gttcactacc tacatcatta tgaaccgttg ggcactgggg aacttagcct gcgacctctg
      601 gctctccatt gactgtgtgg ccagcaatgc ctctgtcatg aatctgctgg tcatcagctt
      661 tgacaggtac ttttccatca cttctccatt ccgctaccag agcctgatga ccagggctcg
      721 agctggtgtg atgattggtc tggcttgggt catctccttt gtcctatggg ctcctgccat
      781 cttgttctgg caatactttg t
//
