LOCUS       sequence-77693-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed mCherry under the control of human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-DIO-mCherry
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(47..80)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(131..164)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      171..180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(322..340)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     590..609
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcataa cttcgtatag
       61 catacattat acgaagttat tctttgcacc attctaaaga ataacagtga taatttctgg
      121 gttaaggcaa ataacttcgt ataggatact ttatacgaag ttatgctagc gccaccatgg
      181 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      241 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      301 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
      361 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
      421 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      481 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
      541 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
      601 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
      661 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
      721 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
      781 tc
//
