LOCUS       sequence-77696-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gq-coupled hM3D DREADD fused with mCherry under the 
            control of EF1a promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-hM3D(Gq)-mCherry
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(132..165)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata
       61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg
      121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag cgccaccatg
      181 accttgcaca ataacagtac aacctcgcct ttgtttccaa acatcagctc ctcctggata
      241 cacagcccct ccgatgcagg gctgcccccg ggaaccgtca ctcatttcgg cagctacaat
      301 gtttctcgag cagctggcaa tttctcctct ccagacggta ccaccgatga ccctctggga
      361 ggtcataccg tctggcaagt ggtcttcatc gctttcttaa cgggcatcct ggccttggtg
      421 accatcatcg gcaacatcct ggtaattgtg tcatttaagg tcaacaagca gctgaagacg
      481 gtcaacaact acttcctctt aagcctggcc tgtgccgatc tgattatcgg ggtcatttca
      541 atgaatctgt ttacgaccta catcatcatg aatcgatggg ccttagggaa cttggcctgt
      601 gacctctggc ttgccattga ctgcgtagcc agcaatgcct ctgttatgaa tcttctggtc
      661 atcagctttg acagatactt ttccatcacg aggccgctca cgtaccgagc caaacgaaca
      721 acaaagagag ccggtgtgat gatcggtctg gcttgggtca tctcctttgt cctttgggct
      781 cctgccatct tgttctggca atactttgtt ggaaagagaa ctgtgcctcc gggagagtgc
      841 ttcattcagt tcctcagtga gcccaccatt acttttggca cagccatcgc tggtttttat
      901 atgcctgtca ccattatgac tattttatac tg
//
