LOCUS       sequence-77697-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gi-coupled hM4D DREADD fused with mCherry under the 
            control of EF1a promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-hM4D(Gi)-mCherry
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..91)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(142..175)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      182..191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctttcacaa attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata
       61 acttcgtata gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg
      121 ataatttctg ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag
      181 cgccaccatg gccaacttca cacctgtcaa tggcagctcg ggcaatcagt ccgtgcgcct
      241 ggtcacgtca tcatcccaca atcgctatga gacggtggaa atggtcttca ttgccacagt
      301 gacaggctcc ctgagcctgg tgactgtcgt gggcaacatc ctggtgatgc tgtccatcaa
      361 ggtcaacagg cagctgcaga cagtcaacaa ctacttcctc ttcagcctgg cgtgtgctga
      421 tctcatcata ggcgccttct ccatgaacct ctacaccgtg tacatcatca agggctactg
      481 gcccctgggc gccgtggtct gcgacctgtg gctggccctg gactgcgtgg tgagcaacgc
      541 ctccgtcatg aaccttctca tcatcagctt tgaccgctac ttctgcgtca ccaagcctct
      601 cacctaccct gcccggcgca ccaccaagat ggcaggcctc atgattgctg ctgcctgggt
      661 actgtccttc gtgctctggg cgcctgccat cttgttctgg cagtttgtgg tgggtaagcg
      721 gacggtgccc gacaaccagt gcttcatcca gttcctgtcc aacccagcag tgacctttgg
      781 cacagccatt gctggcttct acctgcctgt ggtcatcatg acggtgctgt acatccacat
      841 ctccctggcc agtcgcagcc gagtccacaa gcaccggccc gagggcccga aggagaagaa
      901 agccaagacg ctggccttcc tcaagagccc actaatgaag cagagcgtca agaagcccc
//
