LOCUS       sequence-77704-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Coexpresses Trex2 and iRFP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  TITLE     Trex2 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL iRFP-T2A-Trex2
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(764..817)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattga ctaatgcatt caggcttcga
       61 ggcttggacc atcaggtggc acgtacatgg gctcaatatg agcccagctg cgggcctgct
      121 catctgccca ggcgagcagc tcaggagcac gatgcaggaa gatcagaagc agggtgtgca
      181 catcaccttc tgctgaatgg gcagcactgg gttcagcctg gaagtagcgg tggaagagac
      241 tggccaggct gtagcttttg cggccttgag ccctggtgcc gtggctgtga gcacggtcca
      301 ggccccgcaa tgcaggcagt gtgtccaggc agacagtgtc ttggggcaga tgggcaccca
      361 gacgttgtag ctccgtgcac agcagtggga agtcataatc gaagccattg tgggccacaa
      421 ggcagatggg gccctcctgg cggcttagga agccctgcag tgtccttacc acagcgccat
      481 tgaaaccagc cttcccgcag tgcatcaggc tttcgctgct caaaccagta atctcactgg
      541 ccttggcagt aaaggggcgc tccgggcaca tgcacagtgt gagcttgtcc agaacacggg
      601 gcagcaccaa ggaaccagaa tcatcccgtt ctgggttctc cagggaagag cggtgaacag
      661 caaaaaggga tatctctgca atctcagggt ccatgtttgg gagcccagtg gcttctaggt
      721 ccaggaatac aaaggtctca gcccgaggtg gctcagaacc ggtggggccc ggattctcct
      781 ccacgtcacc gcatgttaga agacttcctc tgccctctct agactcttcc atcacgccga
      841 tctgccaggc cagaacctgg gcgactagct cgcaggcttg gcggccatcg agatcgacgt
      901 agtacggcgt tcggtgatgg caaacgatca atccccacag tcgctcgccg cgcaaaatcg
      961 agatcgacat cgtgccgtgc atgcctatgt t
//
