LOCUS       sequence-77713-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination Vector for TALEColor-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-HD(C)-mCherry
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..710)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(278..297)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     547..565
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcagcgcg ttcgtactgt
       61 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag
      121 gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag
      181 tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg
      241 gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg
      301 tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagt
      361 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac
      421 ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg
      481 taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg
      541 ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc
      601 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
      661 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat acttccactt
      721 ccctgcaggc ctgccactcg atgtgatgtc ctctcgggaa tccttctgtt ggtccgctta
      781 atcaatgcag gcgcgtgcgg gagccccttc ttcaccgcat ccaaggcgg
//
