LOCUS       sequence-77719-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for TALEN with TALEColor Backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NI(A)-Fok1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..596)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 tatgagcgga aattgatctc gccattgtta aacttgcgtc tgacttcttc cagtgtcaga
       61 gttcccgctt tgatcatttc tccaccaatg agcagttcct ctacggacaa aaccgcacca
      121 ttgcagttcg taatgtgatt caaccgtgtg agctgggcct tatagttgcc tttgaaatgc
      181 ccagagacaa acaaaaactt aaactcggtc actgatgaag gatacacttt ccaccactca
      241 tttggattga tgtgtttgtt cctggtctga ttttcttcga cataacgttg catctcatct
      301 gcctgcccga tgggaagatt gtacccaccg ctgtaggctt tcgtgtcgac gatcactccg
      361 taatcaatcg ggctccccac cgtatagatt gctccgtcgg gttttcgtga tccaccgaga
      421 tgcttccctc ggtatccata aaccttcata aagaactcca ttaccttcat ctcaaggatt
      481 ctgtcttgag tcgagttcct agcaatttcg ataagttcga tgtattcatg cggtacgtat
      541 ttcaatttgt gccgcagctc cgactttttc tcctcaagtt cactcttcac gagttgggaa
      601 ccacttccct gcaggcctgc cactcgatgt gatgtcctct cgggaatcct tctgttggtc
      661 cgcttaatca atgcaggcgc gtgcgggagc cccttcttca ccgcatccaa ggcgggtcgt
      721 ccgccgaggc aggccagcgc tacaagatga tcattagtga gtgcggccag cgcggggtcc
      781 ggcctcgaaa gctgggccac gattgactcc agtgcgggtc tgcccccgat gttggatgca
      841 a
//
