LOCUS       sequence-77721-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for TALEN with TALEColor Backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NG(T)-Fok1
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..595)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 atgagcggaa attgatctcg ccattgttaa acttgcgtct gacttcttcc agtgtcagag
       61 ttcccgcttt gatcatttct ccaccaatga gcagttcctc tacggacaaa accgcaccat
      121 tgcagttcgt aatgtgattc aaccgtgtga gctgggcctt atagttgcct ttgaaatgcc
      181 cagagacaaa caaaaactta aactcggtca ctgatgaagg atacactttc caccactcat
      241 ttggattgat gtgtttgttc ctggtctgat tttcttcgac ataacgttgc atctcatctg
      301 cctgcccgat gggaagattg tacccaccgc tgtaggcttt cgtgtcgacg atcactccgt
      361 aatcaatcgg gctccccacc gtatagattg ctccgtcggg ttttcgtgat ccaccgagat
      421 gcttccctcg gtatccataa accttcataa agaactccat taccttcatc tcaaggattc
      481 tgtcttgagt cgagttccta gcaatttcga taagttcgat gtattcatgc ggtacgtatt
      541 tcaatttgtg ccgcagctcc gactttttct cctcaagttc actcttcacg agttgggaac
      601 cacttccctg caggcctgcc actcgatgtg atgtcctctc gggaatcctt ctgttggtcc
      661 gcttaatcaa tgcaggcgcg tgcgggagcc ccttcttcac cgcatccaag gcgggtcgtc
      721 cgccgaggca ggccagcgct acaagatgat cattagtgag tgcggccagc gcggggtccg
      781 gcctcgaaag ctgggccacg attgactcca gtgcgggtct gccccctccg ttggatgcaa
      841 tagccactac c
//
