LOCUS       sequence-77722-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bmal full length with cer tag used for mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: LV-CMV-Cer-FL- Bmal L95E
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(59..262)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(88..108)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        263..566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    682..715
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(757..773)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(758..774)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 cccttgctca ccatggtggc gaccggtagc gctagcggat ctgacggttc actaaaccag
       61 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      121 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      181 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      241 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      301 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      361 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
      421 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      481 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      541 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
      601 ccgtaattga ttactattaa taactaatgc atggcggtaa tacggttatc cacgcggccg
      661 cttaagcttg gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt
      721 ccctcgacct gctggaatct cgtgaagcga gcttatcgat accgtcgacc tcgactagag
      781 cgggcccggt actaa
//
