LOCUS       sequence-77723-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Coexpresses Trex2 and iRFP in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Trex2 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pExodus iRFP-T2A-Trex2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(766..819)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 acgggccctc tagactcgag cggccgccac tgtgctggat atctgcagaa ttcaggcttc
       61 gaggcttgga ccatcaggtg gcacgtacat gggctcaata tgagcccagc tgcgggcctg
      121 ctcatctgcc caggcgagca gctcaggagc acgatgcagg aagatcagaa gcagggtgtg
      181 cacatcacct tctgctgaat gggcagcact gggttcagcc tggaagtagc ggtggaagag
      241 actggccagg ctgtagcttt tgcggccttg agccctggtg ccgtggctgt gagcacggtc
      301 caggccccgc aatgcaggca gtgtgtccag gcagacagtg tcttggggca gatgggcacc
      361 cagacgttgt agctccgtgc acagcagtgg gaagtcataa tcgaagccat tgtgggccac
      421 aaggcagatg gggccctcct ggcggcttag gaagccctgc agtgtcctta ccacagcgcc
      481 attgaaacca gccttcccgc agtgcatcag gctttcgctg ctcaaaccag taatctcact
      541 ggccttggca gtaaaggggc gctccgggca catgcacagt gtgagcttgt ccagaacacg
      601 gggcagcacc aaggaaccag aatcatcccg ttctgggttc tccagggaag agcggtgaac
      661 agcaaaaagg gatatctctg caatctcagg gtccatgttt gggagcccag tggcttctag
      721 gtccaggaat acaaaggtct cagcccgagg tggctcagaa ccggtggggc ccggattctc
      781 ctccacgtca ccgcatgtta gaagacttcc tctgccctct ctagactctt ccatcacgcc
      841 gatctgccag gccagaacct gggcgactag ctcgcaggct tggcggccat cgagatcgac
      901 gtagtacggc gttcggtgat ggcaaacgat caatccccac agtcgctcgc cgcgcaaaat
      961 cgagatcgac atcgtgccgt gcatgcctat gttg
//
