LOCUS       sequence-77724-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination Vector for TALEColor-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NI(A)-mCherry
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..709)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(277..296)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     546..564
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcagcgcgt tcgtactgtt
       61 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg
      121 cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt
      181 ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg
      241 ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt
      301 cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcagtg
      361 aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact
      421 tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt
      481 aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc
      541 cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct
      601 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
      661 tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccata cttccacttc
      721 cctgcaggcc tgccactcga tgtgatgtcc tctcgggaat ccttctgttg gtccgcttaa
      781 tcaatgcagg cgcgtgcggg agccccttct tcaccgcatc caaggcgggt cgtccg
//
