LOCUS       sequence-77726-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination Vector for TALEColor-mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NI(A)-mVenus
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(303..608)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(405..424)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aggcctgcca ctcgatgtga tgtcctctcg ggaatccttc tgttggtccg cttaatcaat
       61 gcaggcgcgt gcgggagccc cttcttcacc gcatccaagg cgggtcgtcc gccgaggcag
      121 gccagcgcta caagatgatc attagtgagt gcggccagcg cggggtccgg cctcgaaagc
      181 tgggccacga ttgactccag tgcgggtctg cccccgatgt tggatgcaat agccactacc
      241 tgctcaggcg tgagtcgaga ccgtcgacct gcagactggc tgtgtataag ggagcctgac
      301 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
      361 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
      421 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct
      481 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
      541 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
      601 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg
      661 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
      721 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg
      781 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatac
//
