LOCUS       sequence-77727-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination Vector for TALEColor-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-NH(G)-mCherry
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(434..739)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(536..555)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatacttc
      121 cacttccctg caggcctgcc actcgatgtg atgtcctctc gggaatcctt ctgttggtcc
      181 gcttaatcaa tgcaggcgcg tgcgggagcc ccttcttcac cgcatccaag gcgggtcgtc
      241 cgccgaggca ggccagcgct acaagatgat cattagtgag tgcggccagc gcggggtccg
      301 gcctcgaaag ctgggccacg attgactcca gtgcgggtct gcccccgtgg ttggatgcaa
      361 tagccactac ctgctcaggc gtgagtcgag accgtcgacc tgcagactgg ctgtgtataa
      421 gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt
      481 cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat
      541 cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg
      601 agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg
      661 tgtcaataat atcactctgt acatccacaa acagacgata acggctctct cttttatagg
      721 tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt
      781 tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca
      841 cgcagacgac ggg
//
