LOCUS       sequence-7773-ex        4161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse codon-optimized FLP recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Raymond CS, Soriano P
  TITLE     High-efficiency FLP and PhiC31 site-specific recombination in 
            mammalian cells.
  JOURNAL   PLoS One. 2007 Jan 17;2(1):e162. doi: 10.1371/journal.pone.0000162.
  PUBMED    17225864
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2007-01-17"; volume: "2(1):e162. doi"; pages: " 
            10.1371/journal.pone.0000162"
COMMENT     Sequence Label: pFLPo
FEATURES             Location/Qualifiers
     source          1..4161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1973..1993)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1973..1991)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2012..2028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2012..2028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2025..2047)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2036..2052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2060..2066,2067..2084,2085..2090))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2105..2126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2243..2260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2414..3002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2494..2513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3173..3964,3965..4033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3796..3815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4034..4138)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggctatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccga
      661 tatcgccgcc accatgagcc agttcgacat cctgtgcaag acccccccca aggtgctggt
      721 gcggcagttc gtggagagat tcgagaggcc cagcggcgag aagatcgcca gctgtgccgc
      781 cgagctgacc tacctgtgct ggatgatcac ccacaacggc accgccatca agagggccac
      841 cttcatgagc tacaacacca tcatcagcaa cagcctgagc ttcgacatcg tgaacaagag
      901 cctgcagttc aagtacaaga cccagaaggc caccatcctg gaggccagcc tgaagaagct
      961 gatccccgcc tgggagttca ccatcatccc ttacaacggc cagaagcacc agagcgacat
     1021 caccgacatc gtgtccagcc tgcagctgca gttcgagagc agcgaggagg ccgacaaggg
     1081 caacagccac agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga gcatctggga
     1141 gatcaccgag aagatcctga acagcttcga gtacaccagc aggttcacca agaccaagac
     1201 cctgtaccag ttcctgttcc tggccacatt catcaactgc ggcaggttca gcgacatcaa
     1261 gaacgtggac cccaagagct tcaagctggt gcagaacaag tacctgggcg tgatcattca
     1321 gtgcctggtg accgagacca agacaagcgt gtccaggcac atctactttt tcagcgccag
     1381 aggcaggatc gaccccctgg tgtacctgga cgagttcctg aggaacagcg agcccgtgct
     1441 gaagagagtg aacaggaccg gcaacagcag cagcaacaag caggagtacc agctgctgaa
     1501 ggacaacctg gtgcgcagct acaacaaggc cctgaagaag aacgccccct accccatctt
     1561 cgctatcaag aacggcccta agagccacat cggcaggcac ctgatgacca gctttctgag
     1621 catgaagggc ctgaccgagc tgacaaacgt ggtgggcaac tggagcgaca agagggcctc
     1681 cgccgtggcc aggaccacct acacccacca gatcaccgcc atccccgacc actacttcgc
     1741 cctggtgtcc aggtactacg cctacgaccc catcagcaag gagatgatcg ccctgaagga
     1801 cgagaccaac cccatcgagg agtggcagca catcgagcag ctgaagggca gcgccgaggg
     1861 cagcatcaga taccccgcct ggaacggcat catcagccag gaggtgctgg actacctgag
     1921 cagctacatc aacaggcgga tctgatgaga tatcgagctc cagcttttgt tccctttagt
     1981 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2041 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2101 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2161 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2221 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2281 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2341 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2401 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2461 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2521 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2581 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2641 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2701 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2761 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2821 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2881 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2941 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3001 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3061 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3121 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3181 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3241 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3301 caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3361 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3421 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3481 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3541 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3601 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3661 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3721 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3781 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3841 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3901 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3961 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4021 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4081 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4141 catttccccg aaaagtgcca c
//
