LOCUS       sequence-77731-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid for cloning of megaTALs or other TAL-fusions and 
            expression in mammalian cells. RVDs are cloned between Age-Xho sites
            and meganuclease/fusion is cloned between Xba-Sal sites. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..38
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..89
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             93..113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cccctcactc ggcgcgccta atacgactca ctataggggc cgccaccatg ggatcctgca
       61 ggtatccata tgatgtccca gattatgcgc cacctaagaa gaaacgcaaa gtcgtggatc
      121 tacgcacgct cggctacagt cagcagcagc aagagaagat caaaccgaag gtgcgttcga
      181 cagtggcgca gcaccacgag gcactggtgg gccatgggtt tacacacgcg cacatcgttg
      241 cgctcagcca acacccggca gcgttaggga ccgtcgctgt cacgtatcag cacataatca
      301 cggcgttgcc agaggcgaca cacgaagaca tcgttggcgt cggcaaacag tggtccggcg
      361 cacgcgccct ggaggccttg ctcacggatg cgggggagtt gagaggtccg ccgttacagt
      421 tggacacagg ccaacttgtg aagattgcaa aacgtggcgg cgtgaccgca atggaggcag
      481 tgcatgcatc gcgcaatgca ctgaccggtg catgctagct gactgacctc gagagcattg
      541 tggcccagct gagccggcct gatccggcgt tggccgcgtt gaccaacgac cacctcgtcg
      601 ccttggcctg cctcggcgga cgtcctgcca tggatgcagt gaaaaaggga ttgccgcacg
      661 cgccggaatt gatcagaaga gtcaatcgcc gtattggcga acgcacgtcc catcgcgttg
      721 cgatatctag atgagacgta gtgatctgac gtctcgtcga ccgagcatct taccgccatt
      781 tatacccata tttgttctgt ttttcttgat ttgggtatac atttaaatgt taatagaaca
      841 aaatggtggg gcaatcattt acatttttag ggatatgtaa ttactagttc aggtgtattg
      901 ccacaagaca aacatgttaa gaaactttcc cgttatttac gctctgttcc tgttaatcaa
      961 cctctggatt acaaaatttg tgaaagattg actga
//
