LOCUS       sequence-77732-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid for cloning of megaTALs or other TAL-fusions and 
            expression in mammalian cells. RVDs are cloned between Age-Xho sites
            and meganuclease/fusion is cloned between Xba-Sal sites. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..746
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MASELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             750..803
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             810..836
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             840..860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cccctcactc ggcgcgccag tcctccgaca gactgagtcg cccgatggct agcgagctga
       61 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca
      121 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      181 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg
      241 gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc
      301 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      361 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
      421 acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg
      481 agacactgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg
      541 tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag aaacccgcta
      601 agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg
      661 ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc
      721 ctagcaaact ggggcacaag cttaattcag agggcagagg aagtcttcta acatgcggtg
      781 acgtggagga gaatccgggc ccctgcaggt atccatatga tgtcccagat tatgcgccac
      841 ctaagaagaa acgcaaagtc gtggatctac gcacgctcgg ctacagtcag cagcagcaag
      901 agaagatcaa accgaaggtg cgttcgacag tggcgcagca ccacgaggca ctggtgggcc
      961 atgggtttac acacgcgcac atcgttgcgc tca
//
