LOCUS       sequence-77734-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid for cloning of megaTALs or other TAL-fusions and 
            expression in mammalian cells. RVDs are cloned between Age-Xho sites
            and meganuclease/fusion is cloned between Xba-Sal sites. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(882..902)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(906..932)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(939..992)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt aacaggaaca gagcgtaaat
       61 aacgggaaag tttcttaaca tgtttgtctt gtggcaatac acctgaacta gtaattacat
      121 atccctaaaa atgtaaatga ttgccccacc attttgttct attaacattt aaatgtatac
      181 ccaaatcaag aaaaacagaa caaatatggg tataaatggc ggtaagatgc tcggtcgacg
      241 agacgtcaga tcactacgtc tcatctagat atcgcaacgc gatgggacgt gcgttcgcca
      301 atacggcgat tgactcttct gatcaattcc ggcgcgtgcg gcaatccctt tttcactgca
      361 tccatggcag gacgtccgcc gaggcaggcc aaggcgacga ggtggtcgtt ggtcaacgcg
      421 gccaacgccg gatcaggccg gctcagctgg gccacaatgc tctcgaggtc agtcagctag
      481 catgcaccgg tcagtgcatt gcgcgatgca tgcactgcct ccattgcggt cacgccgcca
      541 cgttttgcaa tcttcacaag ttggcctgtg tccaactgta acggcggacc tctcaactcc
      601 cccgcatccg tgagcaaggc ctccagggcg cgtgcgccgg accactgttt gccgacgcca
      661 acgatgtctt cgtgtgtcgc ctctggcaac gccgtgatta tgtgctgata cgtgacagcg
      721 acggtcccta acgctgccgg gtgttggctg agcgcaacga tgtgcgcgtg tgtaaaccca
      781 tggcccacca gtgcctcgtg gtgctgcgcc actgtcgaac gcaccttcgg tttgatcttc
      841 tcttgctgct gctgactgta gccgagcgtg cgtagatcca cgactttgcg tttcttctta
      901 ggtggcgcat aatctgggac atcatatgga tacctgcagg ggcccggatt ctcctccacg
      961 tcaccgcatg ttagaagact tcctctgccc tct
//
