LOCUS       sequence-77736-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) MND
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(28..52)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ttaatcagct cgctagccat ggtggcggat ctgacggttc actaaacgag ctctgcttat
       61 atagagctcg gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt
      121 caaataaggc acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct
      181 ctagaaactg ctgagggcgg gaccgcatct ggggaccatc tgttcttggc cctgagccgg
      241 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttctgctgtt ccaactgttc
      301 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattctc
      361 ctgtttctct gttcgaattc cggaaacacc cgaatcaact tctagtcaaa ttattgttca
      421 cgccgcaatg acccacccct ggcccgcgtc tgtggaactg acccctggtg tacaggagag
      481 ttcgctgctg aaagtggtcc caaaggggta ctagttttta agctcccaac tccccctccc
      541 ccagcgtctg gaggattcca caccctcgca ccgcaggggc gaggaagtgg gcggagtccg
      601 gttttggcgc cagccgctga ggctgccaag cagaaaagcc accgctgagg agactccggt
      661 cactgtcctc gccccgcctc ccccttccct ccccttgggg accaccgggc gccacgccgc
      721 gaacggtaag tgccgcggtc gtcggcgcct ccgccctc
//
