LOCUS       sequence-77746-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-GFAP-hM4D(Gi)-mCherry
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..759)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(327..346)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     596..614
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      756..765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt acttgtacag
       61 ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt
      121 gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag
      181 ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc
      241 cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg
      301 ctcggaggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa
      361 gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg
      421 ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
      481 ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga
      541 gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt
      601 caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat
      661 ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt
      721 gatgatggcc atgttatcct cctcgccctt gctcaccatg gtggcgaccg gtggatcccg
      781 cctggcagtg ccgatgttcc gatactggca cagcagcagg tgccggaagg tctttttaaa
      841 ggtggcgttg caca
//
