LOCUS       sequence-77747-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of 
            human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(438..471)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(522..555)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(612..633)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctgacgacc gaccccgacc cactggacaa gcacccaacc cccattcccc aaattgcgca
       61 tcccctatca gagaggggga ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc
      121 ttcagcaccg cggacagtgc cttcgccccc gcctggcggc gcgcgccacc gccgcctcag
      181 cactgaaggc gcgctgacgt cactcgccgg tcccccgcaa actccccttc ccggccacct
      241 tggtcgcgtc cgcgccgccg ccggcccagc cggaccgcac cacgcgaggc gcgagatagg
      301 ggggcacggg cgcgaccatc tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc
      361 ggtgggcagc ggaggagtcg tgtcgtgcct gagagcgcag tcgagaaggt accggatcct
      421 ctagagtcga ctccggaata acttcgtata ggatacttta tacgaagtta tgcagaatgg
      481 tagctggatt gtagctgcta ttagcaatat gaaacctctt aataacttcg tatagcatac
      541 attatacgaa gttatggcgc gccctatcac ttgtacagct cgtccatgcc gagagtgatc
      601 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      661 ctcagtttgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      721 atgggggtgt tctgctggta
//
