LOCUS       sequence-77751-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of 
            human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(440..473)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(524..557)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(572..823)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(614..635)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tacctgacga ccgaccccga cccactggac aagcacccaa cccccattcc ccaaattgcg
       61 catcccctat cagagagggg gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca
      121 gcttcagcac cgcggacagt gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc
      181 agcactgaag gcgcgctgac gtcactcgcc ggtcccccgc aaactcccct tcccggccac
      241 cttggtcgcg tccgcgccgc cgccggccca gccggaccgc accacgcgag gcgcgagata
      301 ggggggcacg ggcgcgacca tctgcgctgc ggcgccggcg actcagcgct gcctcagtct
      361 gcggtgggca gcggaggagt cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc
      421 ctctagagtc gactccggaa taacttcgta taggatactt tatacgaagt tatgcagaat
      481 ggtagctgga ttgtagctgc tattagcaat atgaaacctc ttaataactt cgtatagcat
      541 acattatacg aagttatggc gcgccctatc acttgtacag ctcgtccatg ccgagagtga
      601 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      661 tgctcagttt ggactggtag ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      721 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
      781 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atata
//
