LOCUS       sequence-77753-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Magnifico A, Ettenberg S, Yang C, Mariano J, Tiwari S, Fang S, 
            Lipkowitz S, Weissman AM
  TITLE     WW domain HECT E3s target Cbl RING finger E3s for proteasomal 
            degradation.
  JOURNAL   J Biol Chem. 2003 Oct 31. 278(44):43169-77.
  PUBMED    12907674
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2003 Oct 31. 278(44):43169-77."
COMMENT     Sequence Label: pcDNA-HA-Nedd4/2795
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      78..87
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             90..116
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa acgggccctc
       61 tagactcgag cggccgcgcc accatggcct acccatacga cgtcccagac tacgctgagc
      121 tcgggagtgg aatccttacc agcgtgcaga caaaaactat caaaaagtct ttgaatccaa
      181 aatggaatga agaaatactg ttcagggtcc ttccacagcg acaccgcatt cttttcgaag
      241 tgtttgatga aaatcgtttg acaagagatg atttcctagg tcaagtggat gtccctctct
      301 atcctttacc gactgaaaac ccaagaatgg agagaccata tacatttaag gattttgttc
      361 ttcatccaag aagtcacaaa tcaagagtta aaggttatct gagattaaaa atgacttatt
      421 tacctaaaaa tggctcagaa gatgaaaatg cagaccaggc tgaggagtta gagcctggct
      481 gggttgtttt ggaccaacca gatgctgcca ctcatttgcc gcatccacca gaaccctctc
      541 ccctacctcc aggatgggaa gagaggcagg atgtccttgg aaggacctac tacgtaaacc
      601 atgaatctag aagaacacag tggaaaaggc caagccctga cgatgacctc acggatgaag
      661 acaatgatga tatgcagctg caagcgcagc gagcattcac caccaggcgg cagatatcgg
      721 aggatgtgga tggccctgac aaccgggagt cccctgagaa ttgggaaatc gtacgagaag
      781 atgaaaacac cgagtatagt ggtcaggctg tccagtcacc tccatcgggt cacattgatg
      841 tgcagactca ccttgcagaa gagtttaata ccagacttgc cgtgtgtgga aatccagcca
      901 ccagccagcc gg
//
