LOCUS       sequence-77754-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gs-coupled rM3D DREADD fused with mCherry under the 
            control of human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-DIO-rM3D(Gs)-mCherry
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(439..472)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(523..556)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(843..862)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc
       61 atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag
      121 cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca
      181 gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt cccggccacc
      241 ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag
      301 gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg
      361 cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgagaagg taccggatcc
      421 tctagagtcg actccggaat aacttcgtat aggatacttt atacgaagtt atgcagaatg
      481 gtagctggat tgtagctgct attagcaata tgaaacctct taataacttc gtatagcata
      541 cattatacga agttatggcg cgccttactt gtacagctcg tccatgccgc cggtggagtg
      601 gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaggtgat
      661 gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct tggccttgta
      721 ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat
      781 ctcgcccttc agggcgccgt cctcggggta catccgctcg gaggaggcct cccagcccat
      841 ggtcttcttc tgcattacgg ggccgtc
//
