LOCUS       sequence-77760-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Double floxed Gi-coupled hM4D DREADD fused with mCherry under the 
            control of EF1a promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Bryan Roth
  TITLE     Roth lab DREADDs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-DIO-hM4D(Gi)-mCherry
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..71)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(122..155)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(164..874)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(442..461)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     711..729
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtcgtgaggt accggatcct ctagagtcga ctccggaata acttcgtata ggatacttta
       61 tacgaagtta tgcagaatgg tagctggatt gtagctgcta ttagcaatat gaaacctctt
      121 aataacttcg tatagcatac attatacgaa gttatggcgc gccttacttg tacagctcgt
      181 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
      241 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
      301 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
      361 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
      421 aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
      481 tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca
      541 cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga
      601 aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt
      661 acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc ttggtcacct
      721 tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga
      781 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
      841 tggccatgtt atcctcctcg cccttgctca ccatggtg
//
