LOCUS       sequence-77799-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of TRBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Takahashi T, Miyakawa T, Zenno S, Nishi K, Tanokura M, Ui-Tei K
  TITLE     Distinguishable in vitro binding mode of monomeric TRBP and dimeric 
            PACT with siRNA.
  JOURNAL   PLoS One. 2013 May 2;8(5):e63434. doi: 10.1371/journal.pone.0063434.
            Print 2013.
  PUBMED    23658827
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-2"; volume: "8(5):e63434. doi"; pages: " 
            10.1371/journal.pone.0063434. Print 2013"
COMMENT     Sequence Label: pET28a-TRBP
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(71..100)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagctttca cagatcctct tctgagatga gtttttgttc gaaccgcggg ccctctagat
      121 gcatgctcga ggagcttgca gcggccgcct tgctgcctgc catgatcttg aggtactgca
      181 gggcacggcg ggcagcctca ccacgggctg cctccctggt ggttgcagag ccatgacaca
      241 cagtggccgg ctgggtggac agttccacca ggcactggca gagtccactc aggctcagct
      301 cctcaatatc caggtagctg acgtgaaagg cctgctcctc agagagctca ctgaggacac
      361 ggcagcaggc agggcccagg gcacccaggg agcccaggga gcaactgcgg agggacagga
      421 tcttctctcc tactgaattt cgtagagaat cccaggtgca acctgggccc cggtttcgaa
      481 gaccatccag gcgggagccc acaccaatgg agaagtggtc atcatcaggc tccacctcat
      541 tgccatcccg ggcatccaga ggcaccgtgt gcactcgaag cagcattttg gccgccgcat
      601 tccgctttgc caattttttg gaagtgccac tcccaatctc aatgaaacgc tccactcgac
      661 aggtcatggt gaattctttg cggtgggctg gcccagactc ctgggtcact gtgtactccg
      721 gcaaccgcca gcctttctgc accaccagct cctgcagagc accaacgggg ttgcactcag
      781 actgctgagg ggagacaggg ggctgcagtt ccatgggggg gctcctggtt aggactacag
      841 atggaactgg ggtagcagct gctgcagcag taaaaaccgg aatgtcctca
//
