LOCUS       sequence-77800-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of PACT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Takahashi T, Miyakawa T, Zenno S, Nishi K, Tanokura M, Ui-Tei K
  TITLE     Distinguishable in vitro binding mode of monomeric TRBP and dimeric 
            PACT with siRNA.
  JOURNAL   PLoS One. 2013 May 2;8(5):e63434. doi: 10.1371/journal.pone.0063434.
            Print 2013.
  PUBMED    23658827
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-2"; volume: "8(5):e63434. doi"; pages: " 
            10.1371/journal.pone.0063434. Print 2013"
COMMENT     Sequence Label: pET28a-PACT
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(70..99)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tcgggctttg ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc
       61 aagctttcac agatcctctt ctgagatgag tttttgttcg aaccgcgggc cctctagatg
      121 catgctcgag gagcttgcag cggccgcgtc gacctttctt tctgctatta tctttaaata
      181 ctgcaaagca ttgtgagctg catcactttg tgcattgcca caggagatac cggagccatg
      241 acagactgtg atggggctgg tggacagttc agcaagacat tgatattgtc cattggcgct
      301 cagttcatct atatccaaat atgttatatt aaaaccttgt tccttggcaa tttcactaag
      361 cagctggatg taatctgtat ttggaatact aaggaggctt cttttcagta agttgatctt
      421 ttcaccagga gaattcctca aggaatgcca agtacatcct aaagaatgtc ctactacatt
      481 tgttaaagaa atgtggttct ctggagaaat attactaaat ttggcaagaa atttctcagc
      541 agcattcctt ttggcttgct tttttgatgc cccctttcca gtttccataa atgactctag
      601 cctgcaaatt gtagtatatt ctctcttatg agcaggtcct ccctcctggg aaagggtata
      661 ttcaggaagt ctccagccat gatgaatagc caattcctgt aatgaaccaa taggattaag
      721 ctggttcttt ggttgcttgg aagggtcagg cattaagggg tcaggaactg caaagcaaat
      781 acttgcattg gctttcaaaa tgtttatggc agcctctgca gctctatgtt tcgccagctt
      841 cttacttgta ccttcacctg tgcaggttat gtcaccaacg gttactctga aggtgaaagt
      901 gggcacgtgt atttgcacat ca
//
