LOCUS       sequence-77805-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHAGE NFKB-TA-LUC-UBC-dTomato-W.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ, 
            Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN
  TITLE     Lentiviral delivery of RNAi for in vivo lineage-specific modulation 
            of gene expression in mouse lung macrophages.
  JOURNAL   Mol Ther. 2013 Apr;21(4):825-33. doi: 10.1038/mt.2013.19. Epub 2013 
            Feb 12.
  PUBMED    23403494
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2013.19"; journalName: "Mol Ther"; date: "2013-04"; 
            volume: "21"; issue: "4"; pages: "825-33"
COMMENT     Sequence Label: pHAGE NFKB-TA-LUC-UBC-dTomato-W
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     77..95
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(223..254)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    349..466
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(759..803)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcgtaagtga tgtccacctc gatatgtgca tctgtaaaag caattgttcc aggaaccagg
       61 gcgtatctct tcatagcctt atgcagttgc tctccagcgg ttccatcttc cagcggatag
      121 aatggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta
      181 ccggaatgcc aagcttttta ccaacagtac cggaatgcca agctggaatt cgagcttcca
      241 ttatataccc tctagagtct agatctggaa attcccggaa attcccggaa attcccggaa
      301 attcccacta gtccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat
      361 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
      421 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
      481 tgccatttgt gaattcggcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct
      541 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
      601 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
      661 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
      721 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
      781 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
      841 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
      901 atctgttcca gaaatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
      961 a
//
